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BudgetSensonsors AFM Calibration NanogridMon Apr 27 2020

Need a reliable calibration standard for lateral and vertical AFM calibration? Learn more about BudgetSensors CS-20NG nanogrid in our screencast! #AFMProbes #AtomicForceMicroscopy #CalibrationStandard #AFMCalibration https://youtu.be/j7T-IBJ82Mc

Discover how the wear behavior of nanotools high densitiy carbon CDR50-EBD AFM probesThu Apr 23 2020

Pectin Interaction with Immune Receptors is Modulated by Ripening Process in PapayasMon Apr 20 2020

Dietary fibers have been shown to exert immune effects via interaction with pattern recognition receptors (PRR) such as toll-like receptors (TLR) and nucleotide-binding oligomerization domain (NOD)-like receptors. Pectin is a dietary fiber that interacts with PRR depending on its chemical structure. Papaya pectin retains different chemical structures at different ripening stages. How this influences PRR signalling is unknown.* The aim of the article “Pectin Interaction with Immune Receptors is Modulated by Ripening Process in Papayas” by Samira B. R. Prado, Martin Beukema, Eva Jermendi, Henk A. Schols, Paul de Vos and João Paulo Fabi was to determine how ripening influences pectin structures and their ability to interact with TLR2, 3, 4, 5 and 9, and NOD1 and 2.* Papaya ripening is an enzymatic, biochemically driven process that occurs over a short period of time (five days) and involves the mobilization of pectin and the alteration of its chemical composition. The authors evaluated the interaction of the water-soluble fractions rich in pectin extracted from unripe to ripe papayas. The pectin extracted from ripe papayas activated all the TLR and, to a lesser extent, the NOD receptors. The pectin extracted from unripe papayas also activated TLR2, 4 and 5 but inhibited the activation of TLR3 and 9.* During papaya ripening, profound changes in pectin structures lead to differences in the biological effects. The data presented in the paper show that papaya pectin extracted from fruit pulp at different ripening points differently interacted with PRR in a ripening-dependent way. The longer chains of HG from unripe papayas pectin, which were less methyl-esterified, inhibited the activation of TLR3 and 9 and activated TLR2 and 4, in contrast to the ripe papaya’s pectin, which have smaller HG chains with medium methyl esterification thus activating TLR2, 3, 4, 5 and 9.* This variation may represent new biological features of papaya pectin structures in addition to anticancer activities, possibly creating new and cost-effective approaches to extracting papaya pectin with desirable structural and biological features.* These findings might lead to selection of ripening stages for tailored modulation of PRR to support or attenuate immunity in consumers.* The changes in Molecular weight ( Mw ) can also be visualized by Atomic Force Microscopy (see Fig. 1C in the paper.) The AFM images presented in the paper were acquired in tapping mode using an NanoWorld Pointprobe® NCHR AFM probe with a typical spring constant of 42 N/m and typically 320 kHz resonance frequency. The scan speed and scanning resolution were 0.5 Hz and 512 × 512 points, respectively.* https://www.nanoworld.com/pointprobe-tapping-mode-reflex-co… Please have a look at the NanoWorld blog for the full citation and a direct link to the full article. #glycobiology #AFMプローブ #AFMカンチレバー #AFMprobes

Mechanical properties measured by atomic force microscopy define health biomarkers in ageing C. ElegansTue Apr 14 2020

Genetic and environmental factors are key drivers regulating organismal lifespan but how these impact healthspan is less well understood. Techniques capturing biomechanical properties of tissues on a nano-scale level are providing new insights into disease mechanisms.* In the article “ Mechanical properties measured by atomic force microscopy define health biomarkers in ageing C. Elegans “ Clara L. Essmann, Daniel Martinez-Martinez, Rosina Pryor, Kit-Yi Leung, Kalaivani Bala Krishnan, Prudence Pokway Lui, Nicholas D.E. Greene, André E.X. Brown, Vijay M. Pawar, Mandayam A. Srinivasan and Filipe Cabreiro apply Atomic Force Microscopy (AFM) to quantitatively measure the change in biomechanical properties associated with ageing Caenorhabditis elegans in addition to capturing high-resolution topographical images of cuticle senescence.* The authors show that distinct dietary restriction regimes and genetic pathways that increase lifespan lead to radically different healthspan outcomes. Hence, their data support the view that prolonged lifespan does not always coincide with extended healthspan. Importantly, they identify the insulin signalling pathway in C. elegans and interventions altering bacterial physiology as increasing both lifespan and healthspan.* Overall, AFM provides a highly sensitive technique to measure organismal biomechanical fitness and delivers an approach to screen for health-improving conditions, an essential step towards healthy ageing.* The topographical images shown in this article were acquired using NANOSENSORS™ uniqprobe qp-CONT AFM probes. These AFM probes have very soft cantilevers designed for imaging of biological samples ( k = 0.1 N/m ). https://www.nanosensors.com/uniqprobe-uniform-quality-conta… Please have a look at the NANOSENSORS blog for the full citation and a direct link to the full article.#biology #AtomicForceMicroscopy #BiomechanicalProperties #走査型走査型プローブ顕微鏡 #AFMカンチレバー #SPMプローブ

3D‐Printed Scanning‐Probe MicroscopesMon Apr 13 2020

The Microscope That Uses Quantum Physics to Trace AtomsFri Apr 10 2020

SciShow, one of our favorite pop-science YouTube channels recently posted a video about the daddy of the Atomic Force Microscope - the Scanning Tunneling Microscope. For those of you who missed it, here it is. Enjoy! #AtomicForceMicroscopy #AFMProbes #STM #ScanningTunnelingMicroscopy

KPFM surface photovoltage measurement and numerical simulationThu Apr 09 2020

New Post - KPFM surface photovoltage measurement and numerical simulation (https://www.nanoworld.com/…/kpfm-surface-photovoltage-meas…/) has been published on NanoWorld Blog. Kelvin Probe Force Microscopy ( KPFM ) is a scanning probe microscopy technique. It is a combination of the Kelvin probe and of Atomic Force Microscopy methods. The technique consists in evaluating the difference in work function between two conducting materials, by using a nanometer scale tip ( the “KPFMtip”), and placing it close to the material to be characterised, where a difference in work function leads to an electrostatic force developing between the two, which is translated as an oscillation of the tip’s cantilever. A bia sapplied via an external circuit is varied until the force and hence the electrostatic field between sample and KPFM tip is cancelled.* In the article “KPFM surface photovoltage measurement and numerical simulation” Clément Marchat, James P. Connolly, Jean-Paul Kleider, José Alvarez, Lejo J. Koduvelikulathu and Jean Baptiste Puel present a method for the analysis of Kelvin probe force microscopy (KPFM) characterization of semiconductor devices. It enables evaluation of the influence of defective surface layers. The model is validated by analysing experimental KPFM measurements on crystalline silicon samples of contact potential difference (VCPD) in the dark and under illumination, and hence the surface photovoltage (SPV). It is shown that the model phenomenologically explains the observed KPFM measurements. It reproduces the magnitude of SPV characterization as a function of incident light power in terms of a defect density assuming Gaussian defect distribution in the semiconductor bandgap. This allows an estimation of defect densities in surface layers of semiconductors and therefore increased exploitation of KPFM data.* The KPFM measurements were performed using NanoWorld ARROW-EFM conductive AFM tips with a PtIr coating. The tip work function didn’t require calibration because only SPV measurement were performed and studied. Measurements were performed in the KPFM amplitude modulation (AM)mode rather than the frequency modulation (FM) one. The AM mode was chosen because lateral resolution was not a problem on the homogeneous bulk samples studied, allowing focus on the superior surface potential resolution that can be achieved with the AM mode.* https://www.nanoworld.com/electrostatic-force-microscopy-af… Please have a look at the NanoWorld blog for the full citation and a direct link to the full article #surfacevoltage #photovoltaics #KelvinProbeForceMicroscopy #AFMプローブ

DNA looping by two 5-methylcytosine-binding proteins quantified...Mon Mar 30 2020

MeCP2 and MBD2 are members of a family of proteins that possess a domain that selectively binds 5-methylcytosine in a CpG context. Members of the family interact with other proteins to modulate DNA packing. Stretching of DNA–protein complexes in nanofluidic channels with a cross-section of a few persistence lengths allows us to probe the degree of compaction by proteins.* In the article “DNA looping by two 5-methylcytosine-binding proteins quantified using nanofluidic devices” Ming Liu, Saeid Movahed, Saroj Dangi, Hai Pan, Parminder Kaur, Stephanie M. Bilinovich, Edgar M. Faison, Gage O. Leighton, Hong Wang, David C. Williams Jr. and Robert Riehn demonstrate DNA compaction by MeCP2 while MBD2 does not affect DNA configuration. By using atomic force microscopy (AFM), they determined that the mechanism for compaction by MeCP2 is the formation of bridges between distant DNA stretches and the formation of loops.* Despite sharing a similar specific DNA-binding domain, the impact of full-length 5-methylcytosine-binding proteins can vary drastically between strong compaction of DNA and no discernible large-scale impact of protein binding. The authors of the article demonstrate that ATTO 565-labeled MBD2 is a good candidate as a staining agent for epigenetic mapping.* For atomic force microscopy (AFM), the authors used a 7,163-bp linear DNA substrate which contains a 1,697-bp methylated CpG-rich region that is flanked by 2,742-bp and 2,724-bp CpG-free regions. For MeCP2, the DNA substrate and the protein were diluted in AFM imaging buffer (HEPES 20 mM, Mg(OAc)210mM, NaCl 100mM, pH 7.5), mixed together and deposited on freshly peeled mica. For MBD2FLsc, the authors describe how they first mixed the protein and DNA and then diluted the sample in AFM buffer before deposition. The final MeCP2 concentration deposited on mica was 7.5nM, and the MBD2FLsc concentration was 14nM. The mica samples were then washed with filtered deionized water and dried with nitrogen.* NANOSENSORS™ PointProbe® Plus PPP-FMR AFM probes ( ≈2.8N/m) were used to image the sample at a scan resolution of 5.9nm and a scan rate of 3μm/s.* https://www.nanosensors.com/pointprobe-plus-force-modulatio… Please have a look at the NANOSENSORS blog for the full citation and a direct link to the full article. #MeCP2 #DNA #走査型走査型プローブ顕微鏡 #SPMプローブ #AtomicForceMicroscopy #lifescience

Consistent AFM tip shape leading to reproducible results – NANOSENSORS PointProbe® Plus Screencast passes 1000 views markThu Mar 26 2020

The screencast held by Head of R&D Thomas Sulzbach on the NANOSENSORS PointProbe® Plus Silicon AFM probe series with a consistent tip shape leading to more reproducible results has just passed the 1000 views mark. Congratulations Thomas! https://youtu.be/oo-NFxKOko8 Screencasts on the PointProbe® Plus are also available in Japanese https://youtu.be/DczwdZQllD8 https://youtu.be/4eiL8lpQ3fE and in Chinese: https://youtu.be/_o9f_wS7Hj0 also on youku #AtomicForceMicroscopy #AFMプローブ #SPM探针

Self-assembly of small molecules at hydrophobic interfaces using group effectTue Mar 24 2020

Although common in nature, the self-assembly of small molecules at sold–liquid interfaces is difficult to control in artificial systems. The high mobility of dissolved small molecules limits their residence at the interface, typically restricting the self-assembly to systems under confinement or with mobile tethers between the molecules and the surface. Small hydrogen-bonding molecules can overcome these issues by exploiting group-effect stabilization to achieve non-tethered self-assembly at hydrophobic interfaces. Significantly, the weak molecular interactions with the solid makes it possible to influence the interfacial hydrogen bond network, potentially creating a wide variety of supramolecular structures.* In the paper “Self-assembly of small molecules at hydrophobic interfaces using group effect” William Foster, Keisuke Miyazawa, Takeshi Fukuma, Halim Kusumaatmaja and Kislon Voϊtchovsky investigate the nanoscale details of water and alcohols mixtures self-assembling at the interface with graphite through group-effect. They explore the interplay between inter-molecular and surface interactions by adding small amounts of foreign molecules able to interfere with the hydrogen bond network and systematically varying the length of the alcohol hydrocarbon chain. The resulting supramolecular structures forming at room temperature are then examined using atomic force microscopy with insights from computer simulations.* The authors show that the group-based self-assembly approach investigated in the paper is general and can be reproduced on other substrates such as molybdenum disulphide and graphene oxide, potentially making it relevant for a wide variety of systems.* NanoWorld Arrow UHF-AuD ultra high frequency cantilevers for High Speed AFM were used for the amplitude modulation atomic force microscopy described in this paper. https://www.nanoworld.com/ultra-high-frequency-afm-tip-arro… Please have a look at the NanoWorld blog for the full citation and a direct link to the full article. #MolecularSelfAssembly #nanotechnology #FrequencyModulationAtomicForceMicroscopy #ScanningProbeMicroscopy #SelfAssemblyofMolecules #AFMプローブ

MikroMasch® HQ:NSC36 series AFM probesMon Mar 23 2020

Current measurements in the intermittent-contact mode of atomic force microscopy using the Fourier method: a feasibility analysisThu Mar 19 2020

Method for electric current measurements in tapping mode using the ElectriMulti75-G AFM probes based on Fourier analysis https://www.beilstein-journals.org/bjnano/articles/11/37 #AtomicForceMicroscopy #AFMProbes

Highly efficient fullerene and non-fullerene based ternary organic solar cells incorporating a new tetrathiocin-cored semiconductorMon Mar 16 2020

Published new post (Highly efficient fullerene and non-fullerene based ternary organic solar cells incorporating a new tetrathiocin-cored semiconductor) on NANOSENSORS Blog Organic photovoltaic (OPV) devices based on nanocomposites of π-conjugated semiconductors are a prospective solar cell technology and have attracted considerable attention due to unprecedented attributes such as printability, foldability, portability, wearability, semi-transparency and amenability to cost-effective large area fabrication. Extensive and focussed research to enhance the power conversion efficiency (PCE) of organic solar cells have led to the development of highly efficient bulk heterojunction (BHJ) single and multijunction organic solar cells with PCEs > 15%.* In the paper “Highly efficient fullerene and non-fullerene based ternary organic solar cells incorporating a new tetrathiocin-cored semiconductor” Lethy Krishnan Jagadamma, Rupert G. D. Taylor, Alexander L. Kanibolotsky, Muhammad Tariq Sajjad, Iain A. Wright, Peter N. Horton, Simon J. Coles, Ifor D. W. Samuel and Peter J. Skabara present a dual-chain oligothiophene-based organic semiconductor, EH-5T-TTC.* Detailed characterisation of the ternary blend systems as presented in the paper implies that the ternary small molecule EH-5T-TTC functions differently in polymer: fullerene and polymer: non-fullerene blends and has dual functions of morphology modification and complementary spectral absorption.* To understand the differences in recombination dynamics of the blends containing EH-5T-TTC and fullerene/non-fullerene acceptors, detailed morphological characterisation was performed using atomic force microscopy (AFM).* NANOSENSORS™ PointProbe® Plus PPP-NCSTR silicon AFM probes with typical force constant of 7.4 Nm−1 were used for the AFM height images presented in the paper. https://www.nanosensors.com/pointprobe-plus-non-contact-sof… Please have a look at the NANOSENSORS blog for the full citation and a direct link to the full paper #photovoltaics #Fullerene #AtomicForceMicroscopy #AFMプローブ

Flexible 3D Electrodes of Free-Standing TiN Nanotube Arrays Grown by Atomic Layer Deposition with a Ti Interlayer as an Adhesion PromoterMon Mar 09 2020

New post - Flexible 3D Electrodes of Free-Standing TiN Nanotube Arrays Grown by Atomic Layer Deposition with a Ti Interlayer as an Adhesion Promoter (https://www.nanoworld.com/…/flexible-3d-electrodes-of-free…/) has been published on NanoWorld Blog. Nanostructured electrodes and their flexible integrated systems have great potential for many applications, including electrochemical energy storage, electrocatalysis and solid-state memory devices, given their ability to improve faradaic reaction sites by large surface area. Although many processing techniques have been employed to fabricate nanostructured electrodes on to flexible substrates, these present limitations in terms of achieving flexible electrodes with high mechanical stability.* In the study “Flexible 3D Electrodes of Free-Standing TiN Nanotube Arrays Grown by Atomic Layer Deposition with a Ti Interlayer as an Adhesion Promoter” by Seokjung Yun, Sang-Joon Kim, Jaesung Youn, Hoon Kim, Jeongjae Ryu, Changdeuck Bae, Kwangsoo No and Seungbum Hong, the adhesion, mechanical properties and flexibility of TiN nanotube arrays on a Pt substrate were improved using a Ti interlayer. Highly ordered and well aligned TiN nanotube arrays were fabricated on a Pt substrate using a template-assisted method with an anodic aluminum oxide (AAO) template and atomic layer deposition (ALD) system.* The authors show that with the use of a Ti interlayer between the TiN nanotube arrays and Pt substrate, the TiN nanotube arrays could perfectly attach to the Pt substrate without delamination and faceted phenomena. Furthermore, the I-V curve measurements confirmed that the electric contact between the TiN nanotube arrays and substrate for use as an electrode was excellent, and its flexibility was also good for use in flexible electronic devices. Future efforts will be directed toward the fabrication of embedded electrodes in flexible plastic substrates by employing the concepts demonstrated in this study.* The presented strategy provides a new class of nanostructured 3D electrodes to overcome critical mechanical stability, thus providing a great potential platform for application in a flexible integrated device.* Topography and transport properties were investigated using a conductive atomic force microscope with NanoWorld Pointprobe® EFM AFM probes ( Pt-coated conductive AFM tips).* https://www.nanoworld.com/pointprobe-electrostatic-force-mi… Pleae have a look at the NanoWorld blog for the full citation and a direct link to the full article #AtomicForceMicroscopy #NanostructuredMaterials #flexibledevice #ConductiveAFM #SPMプローブ

Infrared nano-spectroscopy of ferroelastic domain walls in hybrid improper ferroelectric Ca3Ti2O7Tue Mar 03 2020

Published new post (Infrared nano-spectroscopy of ferroelastic domain walls in hybrid improper ferroelectric Ca3Ti2O7) on NANOSENSORS Blog Ferroic materials are well known to exhibit heterogeneity in the form of domain walls. Understanding the properties of these boundaries is crucial for controlling functionality with external stimuli and for realizing their potential for ultra-low power memory and logic devices as well as novel computing architectures.* In the article “Infrared nano-spectroscopy of ferroelastic domain walls in hybrid improper ferroelectric Ca3Ti2O7” K. A. Smith, E. A. Nowadnick, S. Fan, O. Khatib, S. J. Lim, B. Gao, N. C. Harms, S. N. Neal, J. K. Kirkland, M. C. Martin, C. J. Won, M. B. Raschke, S.-W. Cheong, C. J. Fennie, G. L. Carr, H. A. Bechtel and J. L. Musfeldt employ synchrotron-based near-field infrared nano-spectroscopy to reveal the vibrational properties of ferroelastic (90∘ ferroelectric) domain walls in the hybrid improper ferroelectric Ca3Ti2O7 . By locally mapping the Ti-O stretching and Ti-O-Ti bending modes, they reveal how structural order parameters rotate across a wall. Thus, they link observed near-field amplitude changes to underlying structural modulations and test ferroelectric switching models against real space measurements of local structure. This initiative opens the door to broadband infrared nano-imaging of heterogeneity in ferroics.* NANOSENSORS™ Platinum Silicide PtSi-NCH AFM probes were used for the Near-field infrared spectroscopy. Atomic force and piezoforce imaging reveal the different orientations of directional order parameters and domain wall character, providing a physical playground for graph theory. * https://www.nanosensors.com/platinum-silicide-non-contact-t… Please have a look at the NANOSENSORS blog for the full citation and a direct link to the full article. #AtomicForceMicroscopy #multiferroics #PiezoresponseForceMicroscopy #AFM

Graphene Quantum Dots as Nanozymes for Electrochemical Sensing of Yersinia enterocolitica in Milk and Human SerumThu Feb 27 2020

New post - Graphene Quantum Dots as Nanozymes for Electrochemical Sensing of Yersinia enterocolitica in Milk and Human Serum (https://www.nanoworld.com/…/graphene-quantum-dots-as-nanoz…/) has been published on NanoWorld Blog. Yersinia enterocolitica is a gram-negative bacillus shaped bacterium that leads to a zootonic disease called yersiniosis. The infection is demonstrated as mesenteric adenitis, acute diarrhea, terminal ileitis, and pseudoappendicitis. Rarely, it can even result in sepsis. According to the 2017 report of the European Food Safety Authority (EFSA) and European Centre for Disease Prevention and Control (ECDC), Y. enterocolitica has been realized as the third most common foodborne-zoonotic disease after campylobacteriosis and salmonellosis in the European Union.* Several studies suggested that #pathogendetection #GrapheneQuantumDots #immunosensor #AtomicForceMicroscopy

Nanotools is attending the SPIE Advanced Lithography in CaliforniaFri Feb 21 2020

BPS 2020 San Diego NanoAndMore booth 818 Thank you and so longThu Feb 20 2020

Thank you all for visiting our booth at the 2020 San Diego Biophysical Society Meeting this week. We hope you have a safe trip home and are looking forward to meeting you again soon https://youtu.be/Q2ubyg6KiOc #bps2020 #biophysics #biomaterials #AFMprobes

Size‐Independent Transmembrane Transporting of Single Tetrahedral...Tue Feb 18 2020

Published new post (Size‐Independent Transmembrane Transporting of Single Tetrahedral DNA Nanostructures) on NANOSENSORS Blog Targeted drug delivery and precision medicine have now become a new paradigm in cancer therapy, with nanocarriers, pharmacologically active drugs could be directly delivered into target cancer cells to manage or reverse the course of disease. Nevertheless, the recent critical issue for drug delivery systems is lack of efficient drug delivery carriers.* DNA nanostructures have attracted considerable attention as drug delivery carriers. However, the transmembrane kinetics of DNA nanostructures remains less explored.* In the article “Size‐Independent Transmembrane Transporting of Single Tetrahedral DNA Nanostructures” Xi Chen, Falin Tian, Min Li, Haijiao Xu, Mingjun Cai, Qian Li, Xiaolei Zuo, Hongda Wang, Xinghua Shi, Chunhai Fan, Huricha Baigude and Yuping Shan describe how they monitored the dynamic process of transporting single tetrahedral DNA nanostructures (TDNs) monitored in real time using a force-tracing technique based on atomic force microscopy.* The authors used special NANOSENSORS™ PointProbe® Plus PPP-BSI AFM probes for the single-molecule force tracing. The NANOSENSORS PPP-BSI AFM tips were modified in two steps as described in their article: First they were washed by Piranha solution (V(H2SO4):V(H2O2) = 3:1) for 1 h, cleaned with ultrapure water twice and absolute ethylacohol once then the AFM tips were dried by argon gas, and cleaned under O3 for 30 min to remove other impurities. After cleaning, the AFM tips were modified with 3-aminopropyltriethoxysilane[17] to generate amino group, it was convenient for linking the heterobifunctional PEG (MAL-PEG2000-SCM, FW≈2000, SensoPathechnologies, Bozeman, MT 1 mg mL−1). After drying with argon, the tips were immersed in a mixture of 100 × 10−9m TDNs, 50 μL NH2OH-reagent (500 × 10−3m NH2OH•HCl, 25 × 10−3m EDTA, pH 7.5), and 50 μL buffer A (100 × 10−3m NaCl, 50 × 10−3m NaH2PO4, 1 × 10−3m EDTA, pH 7.5). After functionalization for 1 h, the AFM tips were washed with PBS for three times and stored at 4 °C .* Please have a look at the NANOSENSORS blog for the full citation and a direct link to the full article #forcetracing #AtomicForceMicroscopy #DNAnanostructures #bsp2020

It’s the last day @BiophysicalSoc Meeting 2020 exhibit in San DiegoTue Feb 18 2020