Millisecond dynamics of an unlabeled amino acid transporter.Sat Dec 05 2020
In the article “Millisecond dynamics of an unlabeled amino acid transporter “ Tina R. Matin, George R. Heath, Gerard H. M. Huysmans, Olga Boudker and Simon Scheuring develop and apply high-speed atomic force microscopy line-scanning (HS-AFM-LS) combined with automated state assignment and transition analysis for the determination of transport dynamics of unlabeled membrane-reconstituted GltPh, a prokaryotic EAAT homologue, with millisecond temporal resolution.
Thank you all for visiting us at our virtual booth at the 2020 Virtual MRS Spring/Fall Meeting & Exhibit.Fri Dec 04 2020
Thank you all for visiting us at our virtual booth at the 2020 Virtual MRS Spring/Fall Meeting & Exhibit. Although the Zoom meeting function will not be active anymore you can still have a look at all our #AFMprobe exhibits for a while.
We hope we will be able to meet you face to face again next fall.
Take care of yourselves and stay healthy!
Nanotools EBD-HAR applied to determine the thickness and roughness of graphene oxide films.Thu Dec 03 2020
Discover how nanotools EBD-HAR #AFMprobes https://www.nanoandmore.com/AFM-Probe-EBD-HAR are applied to determine the thickness and roughness of graphene oxide films in the latest nanotools blogpost https://www.nanotools.com/blog/index.html
Don't miss your chance to visit @NanoAndMore booth at the virtual MRS Spring/Fall 2020 MeetingTue Dec 01 2020
Don't miss your chance to visit @NanoAndMore booth at the virtual MRS Spring/Fall 2020 Meeting, 27 Nov to 4 Dec. @Materials_MRS to find out about free #AFMprobes samples and other goodies we have in store for you.
Millisecond dynamics of an unlabeled amino acid transporterMon Nov 30 2020
Excitatory amino acid transporters (EAATs) are important in many physiological processes and crucial for the removal of excitatory amino acids from the synaptic cleft.*
In the article “Millisecond dynamics of an unlabeled amino acid transporter “ Tina R. Matin, George R. Heath, Gerard H. M. Huysmans, Olga Boudker and Simon Scheuring develop and apply high-speed atomic force microscopy line-scanning (HS-AFM-LS) combined with automated state assignment and transition analysis for the determination of transport dynamics of unlabeled membrane-reconstituted GltPh, a prokaryotic EAAT homologue, with millisecond temporal resolution.*
Among the bulk and single-molecule techniques, high-speed atomic force microscopy ( HS-AFM ) stands out with its ability to provide real-time structural and dynamical information of single molecules. HS-AFM images label-free molecules under close-to-physiological conditions with ~0.1 nm vertical and ~1 nm lateral imaging resolution. Furthermore, HS-AFM has typically ~100 ms temporal resolution, giving access to structure–dynamics relationship of proteins, though the achievable imaging speed depends on sample characteristics like scan size and surface corrugation.
#SingleMoleculeBiophysics #AminoAcidTransporter #videorateAFM #高速AFMカンチレバー #高速原子力显微镜 #HSAFMLS
https://www.nanoworld.com/blog/millisecond-dynamics-of-an-unlabeled-amino-acid-transporter/
NanoAndMore proudly participates in the virtual MRS Spring/Fall 2020 MeetingFri Nov 27 2020
NanoAndMore proudly participates in the virtual MRS Spring/Fall 2020 Meeting, 27 Nov to 4 Dec. @Materials_MRS Visit us at our virtual booth and check out what goodies we have in store for you.
Nanoscale Charge Accumulation and Its Effect on Carrier Dynamics in Tri-cation Perovskite StructuresThu Nov 26 2020
Nanoscale investigations by scanning probe microscopy have provided major contributions to the rapid development of organic–inorganic halide perovskites (OIHP) as optoelectronic devices. Further improvement of device level properties requires a deeper understanding of the performance-limiting mechanisms such as ion migration, phase segregation, and their effects on charge extraction both at the nano- and macroscale.
In the article “Nanoscale Charge Accumulation and Its Effect on Carrier Dynamics in Tri-cation Perovskite Structures” David Toth, Bekele Hailegnaw, Filipe Richheimer, Fernando A. Castro, Ferry Kienberger, Markus C. Scharber, Sebastian Wood and Georg Gramse describe how they studied the dynamic electrical response of Cs0.05(FA0.83MA0.17)0.95PbI3–xBrx perovskite structures by employing conventional and microsecond time-resolved open-loop Kelvin probe force microscopy (KPFM).
A 1940s stylus profilerMon Nov 23 2020
A 1940s stylus profiler. This is the instrument scientists used to analyze surface topography half a century before the Atomic Force Microscope was invented in 1985.
MikroMasch® HQ:NSC14/Al BS probes used in a recent studyWed Nov 11 2020
Our MikroMasch® HQ:NSC14/Al BS probes are used in this recent study of the interruptions in the triple-helix-defining sequence along the collagenous domain in collagen IV and their impact on its flexibility.
We ‘re celebrating the World Science Day for Peace and Development today!Tue Nov 10 2020
We ‘re celebrating the World Science Day for Peace and Development today
This year the focus of World Science Day is on “Science for and with Society in dealing with the global pandemic.”
Keep up the good work!
Polyaleuritate coatings for food metal packaging analyzed with BudgetSensors® SiNi AFM probesMon Nov 02 2020
Polyaleuritate coatings for food metal packaging inspired by biopolyester plant cutin analyzed with BudgetSensors SiNi AFM probes
Nanotools EBD-HAR probes featured in research articleWed Oct 28 2020
Discover how nanotools hydrophobic and chemical resistant biosphere™ #AFMprobes with 50 nm radius are applied for nanoindentation of MPN films enabling reduction of adhesion issues between tip and films.
NanoAndMore proudly sponsors the #AVS67 Virtual Showcase this weekTue Oct 27 2020
NanoAndMore proudly sponsors the #AVS67 Virtual Showcase this week. It is being held October 27-29 10:00 am-1:15 pm. (EDT) and covers a range of emerging topics related to #materialsprocessing and interfaces in the research and manufacturing communities. Live sessions ( the first one starts at 10 a.m. EDT today ) are available and participation is free of charge. Haven’t registered yet? Have a look at: https://avs67.avs.org
Molecular and nanoscale evaluation of N-cadherin expression in invasive bladder cancer cells under control conditions or GW501516 exposureMon Oct 26 2020
In the article “Molecular and nanoscale evaluation of N-cadherin expression in invasive bladder cancer cells under control conditions or GW501516 exposure” Céline Elie-Caille, Isabelle Lascombe, Adeline Péchery, Hugues Bittard and Sylvie Fauconnet, describe how they aimed at exploring the expression level of N-cadherin in invasive bladder cancer cells upon GW501516 exposure by both molecular biology techniques such as RTqPCR and Western blotting and Atomic Force Microscopy ( AFM ) using NanoWorld Pyrex-Nitride silicon nitride PNP-TR AFM tips functionalized with a monoclonal antibody directed against this adhesion molecule. *
Please have a look at the NanoWorld® blog for the full citation and a direct link to the full article.
Yeast Nanometric Scale Oscillations Highlights Fibronectin Induced Changes in C. AlbicansMon Oct 19 2020
Yeast resistance to antifungal drugs is a major public health issue. Fungal adhesion onto the host mucosal surface is still a partially unknown phenomenon that is modulated by several actors among which fibronectin plays an important role. Targeting the yeast adhesion onto the mucosal surface could lead to potentially highly efficient treatments. *
In the article “Yeast Nanometric Scale Oscillations Highlights Fibronectin Induced Changes in C. Albicans” Anne-Céline Kohler, Leonardo Venturelli, Abhilash Kannan, Dominique Sanglard, Giovanni Dietler, Ronnie Willaert and Sandor Kasas, explore the effect of fibronectin on the nanomotion pattern of different Candida albicans strains by atomic force microscopy ( AFM )-based nanomotion detection and correlated the cellular oscillations to the yeast adhesion onto epithelial cells. *
Please have a look at the NANOSENSORS blog for the full citation and a direct link to the full article.
Electrochromic switching of tungsten oxide films grown by reactive ion-beam sputter depositionTue Oct 13 2020
The building sector and within it the class of so-called smart windows plays an important role in terms of energy saving potential. Chromogenic thin films are crucial building blocks in smart windows to modulate the flux of visible light and heat radiation into buildings.
In “Electrochromic switching of tungsten oxide films grown by reactive ion-beam sputter deposition” Mario Gies et al. describe how Tungsten oxide thin films were grown by ion-beam sputter deposition (IBSD) and then show the possibility of influencing samples characteristics by using different preparation parameters. This allows to tune the elemental composition, optical properties or to influence the structure and the degree of crystallization in the resulting thin films.
NanoWorld® Pointprobe® SEIHR AFM probes designed for soft non-contact mode imaging were used for the AFM investigations described in the cited article. https://www.nanoworld.com/pointprobe-seiko-instruments…
Please have a look at the NanoWorld blog for the full citation and a link to the full article
https://www.nanoworld.com/blog/electrochromic-switching-of-tungsten-oxide-films-grown-by-reactive-ion-beam-sputter-deposition/
https://www.nanoworld.com/blog/electrochromic-switching-of-tungsten-oxide-films-grown-by-reactive-ion-beam-sputter-deposition/
How does this technique work with Atomic Force Microscopy and ångström?Thu Oct 08 2020
How does this technique work with Atomic Force Microscopy and ångström?
Multi-Channel Exploration of O Adatom on TiO2(110) Surface by Scanning Probe MicroscopyTue Oct 06 2020
In the article “Multi-Channel Exploration of O Adatom on TiO2(110) Surface by Scanning Probe Microscopy” Huan Fei Wen, Yasuhiro Sugawara and Yan Jun describe how they studied the O2 dissociated state under the different O2 exposed temperatures with atomic resolution by scanning probe microscopy (SPM) and imaged the O adatom by simultaneous atomic force microscopy (AFM)/scanning tunneling microscopy (STM).*
The effect of AFM operation mode on O adatom contrast was investigated, and the interaction of O adatom and the subsurface defect was observed by AFM/STM. Multi-channel exploration was performed to investigate the charge transfer between the adsorbed O and the TiO2(110) by obtaining the frequency shift, tunneling current and local contact potential difference at an atomic scale. The tunneling current image showed the difference of the tunneling possibility on the single O adatom and paired O adatoms, and the local contact potential difference distribution of the O-TiO2(110) surface institutively revealed the charge transfer from TiO2(110) surface to O adatom. The experimental results are expected to be helpful in investigating surface/interface properties by SPM. *
Iridium-coated ultrastiff AFM cantilever SD-T10L100 from the NANOSENSORS Special Developments List (typical Force constant 2 000 N/m) were used in the presented study.
The cantilever tip was first degassed at approximately 650 K for 30 min and then cleaned by Ar ion bombardment to remove the contaminants, prior to the measurements. Features of the surface structure were related to the charge states of the tip apex, and a stable tip was essential to accurately characterize the surface structure and properties in the experiment. The imaging mode became stable in AFM experiments when the metal-coated Si cantilever was employed in the experiments. *
Please have a look at the NANOSENSORS blog for the full citation and a direct link to the full article.
BudgetSensors® diamond-coated All-In-One-DD AFM probes used in a recent studyTue Oct 06 2020
Extracting indentation modulus at the nanoscale via white-noise excitation in Stochastic Atomic Force Acoustic Microscopy (S-AFAM) with the help of BudgetSensors® diamond-coated All-In-One-DD AFM probes.
Nanotools tilt-corrected M-CIS applied to measure Patterned Sapphire SubstrateThu Oct 01 2020
Discover how very durable nanotools tilt-corrected M-CIS #AFMprobes with 400 nm length and 4˚ half cone angle https://www.nanotools.com/…/high-aspect-ratio/m-cis.html are applied to measure height, diameters, pitch, and side angles of Patterned #SapphireSubstrate ( PSS ) in the latest nanotools blogpost. https://www.nanotools.com/blog/index.html
Highlights
AFM Probe Focus
qp-HBC
uniqprobe™ - HeartBeat Cantilever for ScanAsyst®** and Peak Force Tapping™**
Coating:
Reflective Aluminum
Tip Shape: Circular symmetric
Tip Shape: Circular symmetric
AFM Cantilever:
F
60 kHz
C
0.5 N/m
L
115 µm
ATEC-NC
Tapping Mode AFM Probe with REAL Tip Visibility
Coating:
none
Tip Shape: Visible
Tip Shape: Visible
AFM Cantilever:
F
335 kHz
C
45 N/m
L
160 µm
TESPA
Standard Tapping Mode AFM Probe
Coating:
Reflective Aluminum
Tip Shape: Standard
Tip Shape: Standard
AFM Cantilever:
F
320 kHz
C
42 N/m
L
125 µm
OTESPA
Standard Tapping Mode AFM Probe with AFM Tip at the Very End of the AFM Cantilever
Coating:
Reflective Aluminum
Tip Shape: Optimized Positioning
Tip Shape: Optimized Positioning
AFM Cantilever:
F
300 kHz
C
26 N/m
L
160 µm
