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The exhibit @BiophysicalSoc Meeting 2020 in San Diego will be open from 10 am todaySun Feb 16 2020

Booth #818 Biophysical Society Annual Meeting 2020 San Diego NanoAndMoreSun Feb 16 2020

https://youtu.be/qhJaUJ2E3mQ We’re all set up and ready to welcome you @BiophysicalSoc Annual Meeting 2020 in San Diego! Don't forget to visit us and pick up your free sample of NANOSENSORS uniqprobe qp-BioT #AFMprobes at NanoAndMore USA booth #818 during the next three days.https://www.nanosensors.com/uniqprobe-uniform-quality-conta… We're looking forward to welcome you! #bps2020 #biomaterials #biophysics #molecularbiology

Researchers at KRISS applied nanotools CDR30-EBDThu Feb 13 2020

A blast from the pastMon Feb 10 2020

Optical manipulation of sphingolipid biosynthesis using photoswitchable ceramidesMon Feb 10 2020

New post - Optical manipulation of sphingolipid biosynthesis using photoswitchable ceramides (https://www.nanoworld.com/…/optical-manipulation-of-sphing…/) has been published on NanoWorld Blog. Ceramides are central intermediates of sphingolipid metabolism that also function as potent messengers in stress signaling and apoptosis. Progress in understanding how ceramides execute their biological roles is hampered by a lack of methods to manipulate their cellular levels and metabolic fate with appropriate spatiotemporal precision.* In the article “Optical manipulation of sphingolipid biosynthesis using photoswitchable ceramides” Matthijs Kol, Ben Williams, Henry Toombs-Ruane, Henri G Franquelim, Sergei Korneev, Christian Schroeer, Petra Schwille, Dirk Trauner, Joost CM Holthuis and James A Frank report on clickable, azobenzene-containing ceramides, caCers, as photoswitchable metabolic substrates to exert optical control over sphingolipid production in cells.* They combine atomic force microscopy on model bilayers with metabolic tracing studies in cells, and demonstrate that light-induced alterations in the lateral packing of caCers lead to marked differences in their metabolic conversion by sphingomyelin synthase and glucosylceramide synthase. These changes in metabolic rates are instant and reversible over several cycles of photoswitching. The findings described in the article disclose new opportunities to probe the causal roles of ceramides and their metabolic derivatives in a wide array of sphingolipid-dependent cellular processes with the spatiotemporal precision of light.* The High-speed AFM in AC mode described in the article was done with NanoWorld Ultra-Short Cantilevers USC-F0.3-k0.3 with a typical stiffness of 0.3 N/m. The AFM cantilever oscillation was tuned to a frequency of 100–150 kHz and the amplitude kept below 10 nm. The scan rate was set to 25–150 Hz. Images were acquired at 256 × 256 pixel resolution. All measurements were performed at room temperature. The force applied on the sample was minimized by continuously adjusting the set point and gain during imaging. Height, error, deflection and phase-shift signals were recorded and images were line-fitted as required.* https://www.nanoworld.com/Ultra-Short-Cantilevers-USC-F0.3-… Please have a look at the NanoWorld blog for the full citation and a direct link to the full article. #CellBiology #sphingosine #BioactiveLipids #AFMprobes #HighSpeedAtomicForceMicroscopy

Today is the last day at the Linz Winter WorkshopMon Feb 03 2020

3D Superparamagnetic Scaffolds for Bone Mineralization under Static Magnetic Field StimulationMon Feb 03 2020

Published new post (3D Superparamagnetic Scaffolds for Bone Mineralization under Static Magnetic Field Stimulation) on NANOSENSORS Blog Bone is the second most commonly transplanted tissue, preceded only by blood transfusion.Within this context, it is imperative to achieve the functional and structural restoration of damaged bone tissue. A major difficulty in bone tissue engineering arises from the fact that the bone regeneration process requires a long time for achieving a completely functional tissue. Generally, cells are seeded ex vivo into a three-dimensional (3D) biocompatible and sometimes biodegradable structure called scaffold, where they attach and grow. After the implantation into the injured site, the scaffolds should allow proper host cell colonization for regeneration purposes.* Magnetic scaffolds emerged as promising solution for this purpose. Activation of the magnetic scaffolds using external static magnetic fields (SMF) prevents the decrease of bone mineral density and promotes the bone regeneration in bone fractures. The significant alterations in cell behaviors stimulated by the externally applied magnetic fields has been demonstrated in numerous studies. For example, it has been shown that an externally applied SMF using a magnet accelerates the osteogenic differentiation of osteoblasts-like cells in vitro and triggers peri-implant bone formation in vivo.* The magnetism can also be used through scaffolding materials with magnetic properties. For example, biomaterials that incorporate magnetic nanoparticles (MNPs) are being developed.* In the article «3D Superparamagnetic Scaffolds for Bone Mineralization under Static Magnetic Field Stimulation” Irina Alexandra Paun, Bogdan Stefanita Calin, Cosmin Catalin Mustaciosu, Mona Mihailescu, Antoniu Moldovan, Ovidiu Crisan, Aurel Leca and Catalin Romeo Luculescu report on three-dimensional (3D) superparamagnetic scaffolds that enhanced the mineralization of magnetic nanoparticle-free osteoblast cells. The scaffolds were fabricated with submicronic resolution by laser direct writing via two photons polymerization of Ormocore/magnetic nanoparticles (MNPs) composites and possessed complex and reproducible architectures.* To prove the magnetic nature of the MNPs in the polymerized composites, magnetic force microscopy (MFM) was carried out on scaffolds with different MNPs concentrations. NANOSENSORS™ PointProbe® Plus PPP-MFMR AFM probes with magnetic coating were used.* https://www.nanosensors.com/pointprobe-plus-magnetic-force-… Please have a look at the NANOSENSORS blog for the full citation and a direct link to the full article. #ExtracellularMatrixMineralization #MFM #SuperparamagneticScaffold #AFMprobes

The free energy landscape of retroviral integrationTue Jan 28 2020

New post - The free energy landscape of retroviral integration (https://www.nanoworld.com/…/the-free-energy-landscape-of-r…/) has been published on NanoWorld Blog.

Retroviral integration, the process of covalently inserting viral DNA into the host
genome, is a point of no return in the replication cycle. Yet, strand transfer
is intrinsically iso-energetic and it is not clear how efficient integration
can be achieved.*

In the article “The free energy landscape of retroviral integration” published in Nature Communications Willem Vanderlinden, Tine Brouns, Philipp U. Walker, Pauline J. Kolbeck, Lukas F. Milles, Wolfgang Ott, Philipp C. Nickels, Zeger Debyser and Jan Lipfert use biochemical assays, atomic force microscopy (AFM), and multiplexed single-molecule magnetic tweezers (MT) to study tetrameric prototype foamy virus (PFV) strand-transfer dynamics.*

Their finding that PFV intasomes employ auxiliary-binding sites for modulating the barriers to integration raises the question how the topology of higher-order intasomes governs integration of pathogenic retroviruses, most notably HIV. The single-molecule assays developed in this work are expected to be particularly useful to further unravel the complexity of this important class of molecular machines.*

The AFM images were recorded in amplitude modulation mode under ambient conditions and by using NanoWorld high resolution SuperSharpSilicon™ SSS-NCH cantilevers ( resonance frequency ≈300 kHz; typical end-radius 2 nm; half-cone angle <10 deg). Typical scans were recorded at 1–3 Hz line frequency, with optimized feedback parameters and at 512 × 512 pixels.*
https://www.nanoworld.com/pointprobe-super-sharp-silicon-ta…
https://youtu.be/qcS-d6qJFrQ

Please have a look at the NanoWorld blog for the full citation and a direct link to the full article.
#EnzymeMechanisms #AtomicForceMicroscopy #AFMプローブ #走査型走

Happy New Lunar YearFri Jan 24 2020

New post - Happy New Lunar Year (https://www.nanoworld.com/blog/happy-new-lunar-year-5/) has been published on NanoWorld Blog. We wish everyone a good start into the new lunar year of the rat. (Not only the lab rats!) The lab rat is expecting great research results this lunar year. #ChineseNewYear2020 #yearofmouse2020 #ねずみ年 #恭喜发财 #AtomicForceMicroscopy

Happy New Lunar Year!Thu Jan 23 2020

Published new post (Happy New Lunar Year!) on NANOSENSORS™ Blog We wish everyone a good start into the year of the rat! NANOSENSORS™ wishes you all a happy and successful year of the rat #LunarNewYear2020 #yearofmouse #ねずみ年 #鼠年 #AFMプローブ #AFM

We are thrilled to present our brand new BudgetSensors® KPFM & EFM Sample!Wed Jan 22 2020

Kelvin probe force microscopy work function characterization of transition metal oxide crystals under ongoing reduction and oxidationMon Jan 20 2020

Published new post (Kelvin probe force microscopy work function characterization of transition metal oxide crystals under ongoing reduction and oxidation) on NANOSENSORS™ Blog Controlling the work function of transition metal oxides is of key importance with regard to future energy production and storage. As the majority of applications involve the use of heterostructures, the most suitable characterization technique is Kelvin probe force microscopy (KPFM), which provides excellent energetic and lateral resolution.* In their study “Kelvin probe force microscopy work function characterization of transition metal oxide crystals under ongoing reduction and oxidation» Dominik Wrana, Karol Cieślik, Wojciech Belza, Christian Rodenbücher, Krzysztof Szot and Franciszek Krok present the advantages and limitations of the FM-KPFM technique using the example of a newly discovered TiO/SrTiO3(100) (metal/insulator) heterostructure, which has potentially high technological relevance.* In the same article a combined conductivity and work function study from the same surface area is presented, showing the possibility of obtaining full information on the electronic properties when the KPFM technique is accompanied by local conductivity atomic force microscopy (LC-AFM).* The authos present the measurement of the crystalline TiO work function and its dependence on the gaseous pressure of air using Kelvin probe force microscopy. In order to ensure reproducible FM-KPFM results, two different types of AFM cantilevers were used: NANOSENSORS™ PointProbe® Plus PPP-ContPt (PtIr-coated) https://www.nanosensors.com/pointprobe-plus-contact-mode-pt… and NANOSENSORS™ Platinum Silicide PtSi-FM.* https://www.nanosensors.com/platinum-silicide-force-modulat… Such cantilevers are widely used as conducting tips in a contact mode AFM, allowing for a high lateral resolution in conductivity measurements. The remarkable mechanical stability of the selected cantilevers allowed for the noncontact mode measurements (with a Kelvin loop) using the very same tip, maintaining oscillations at the higher harmonics of the fundamental frequency (≈75 kHz). Hence, in order to record current and CPD maps from the very same sample area, KPFM measurements were first performed with the soft cantilever forced to oscillate at higher harmonics, then the tip was retracted tens of nanometers from the surface, all feedback loops were turned down and a contact mode AFM scan was performed when approached with a single loop maintaining a deflection set point of 10–30 mV. The high conductivity of both TiO and STO materials enabled a low sample bias of +1 mV for the LC-AFM measurements to be used.* Please have a look at the NANOSENSORS blog for the full citation and a direct link to the full article. #KPFM #ScanningKelvinProbeMicroscopy #SrTiO3 #原子間力顕微鏡 #AFMプローブ

More papers on High Speed Atomic Force Microscopy – list of references updatedTue Jan 14 2020

New post - More papers on High Speed Atomic Force Microscopy – list of references updated (https://www.nanoworld.com/…/more-papers-on-high-speed-atom…/) has been published on NanoWorld Blog. We have updated our list of articles in the field of High-Speed AFM (HS-AFM) on the www.highspeedscanning.com website. If you would like to see what has been going on recently in the field of High-Speed AFM (HS-AFM) then you are welcome to have a look at: http://www.highspeedscanning.com/hs-afm-references.html We are aware that this list is far from complete so if you have used one of our Ultra-Short Cantilevers (USC) for high speed atomic force microscopy in the research for your publication and your article isn’t listed yet then please let us know. We will be happy to add it to the list. #ScanningProbeMicroscopy #UltrafastScanning #highspeedAFM #molecularbiology #lifescience

Real time dynamics of Gating-Related conformational changes in CorAMon Jan 13 2020

New post - Real time dynamics of Gating-Related conformational changes in CorA (https://www.nanoworld.com/…/real-time-dynamics-of-gating-r…/) has been published on NanoWorld® Blog. Magnesium (Mg2+) is a key divalent cation in biology. It regulates and maintains numerous, physiological functions such as nucleic acid stability, muscle contraction, heart rate and vascular tone, neurotransmitter release, and serves as cofactor in a myriad of enzymatic reactions. Most importantly, it coordinates with ATP, and is thus crucial for energy production in mitochondria.* In order to store Mg2+ in the mitochondrial lumen it is imported via Mrs2 and Alr2 ion channels that are closely related to CorA, the main Mg2+-importer in bacteria. Although these Mg2+-transport proteins do not show much sequence conservation, they all share two trans-membrane domains (TMDs) with the signature motif Glycine-Methionine-Asparagine (GMN) at the extracellular loop.* CorA, a divalent-selective channel in the metal ion transport superfamily, is the major Mg2+-influx pathway in prokaryotes. CorA structures in closed (Mg2+-bound), and open (Mg2+-free) states, together with functional data showed that Mg2+-influx inhibits further Mg2+-uptake completing a regulatory feedback loop. While the closed state structure is a symmetric pentamer, the open state displayed unexpected asymmetric architectures.* In the article “Real time dynamics of Gating-Related conformational changes in CorA” Martina Rangl, Nicolaus Schmandt, Eduardo Perozo and Simon Scheuring used high-speed atomic force microscopy (HS-AFM), to explore the Mg2+-dependent gating transition of single CorA channels: HS-AFM movies during Mg2+-depletion experiments revealed the channel’s transition from a stable Mg2+-bound state over a highly mobile and dynamic state with fluctuating subunits to asymmetric structures with varying degree of protrusion heights from the membrane.* Their data shows that at Mg2+-concentration below Kd, CorA adopts a dynamic (putatively open) state of multiple conformations that imply structural rearrangements through hinge-bending in TM1. They also discuss how these structural dynamics define the functional behavior of this ligand-dependent channel.* All Atomic Force Microscopy experiments described in the article were performed using NanoWorld Ultra-Short Cantilevers USC-F1.2-k0.15 for high-speed Atomic Force Microscopy ( HS-AFM ). Videos of CorA membranes were recorded with imaging rates of ~1–2 frames s−1 and at a resolution of 0.5 nm pixel−1. https://www.nanoworld.com/Ultra-Short-Cantilevers-USC-F1.2-… Please have a look at the NanoWorld blog for the full citation and a direct link to the full article. #molecularbiology #videorateAFM #AtomicForceMicroscopy

MikroMasch® New PosterThu Jan 09 2020

Discover new worlds at the nanoscale with MikroMasch® AFM Probes! Get our new 2020 free poster here: https://www.spmtips.com/ #AtomicForceMicroscopy #AFMProbe

On-chip integration of single solid-state quantum emitters with a SiO2 photonic platformWed Jan 08 2020

Published new post (On-chip integration of single solid-state quantum emitters with a SiO2 photonic platform) on NANOSENSORS™ Blog One important building block for future integrated nanophotonic devices is the scalable on-chip interfacing of single photon emitters and quantum memories with single optical modes.* In the article “On-chip integration of single solid-state quantum emitters with a SiO2 photonic platform” Florian Böhm, Niko Nikolay, Christoph Pyrlik, Jan Schlegel, Andreas Thies, Andreas Wicht,Günther Tränkle and Oliver Benson present the deterministic integration of a single solid-state qubit, the nitrogen-vacancy (NV) center, with a photonic platform consisting exclusively of SiO2grown thermally on a Si substrate.* The platform stands out by its ultra-low fluorescence and the ability to produce various passive structures such as high-Q micro resonators and mode-size converters. By numerical analysis an optimal structure for the efficient coupling of a dipole emitter to the guided mode could be determined. Experimentally, the integration of a preselected NV emitter was performed with an atomic force microscope and the on-chip excitation of the quantum emitter as well as the coupling of single photons to the guided mode of the integrated structure could be demonstrated. The authors approach shows the potential of this platform as a robust nanoscale interface of on-chip photonic structures with solid-state qubits.* After optically verifying the successful placement of the nanodiamond hosting a single nitrogen-vacancy ( NV ) center by performing a confocal scan, the article describes how the nanoparticle is pushed to the inner edge of the rib waveguide in a subsequent step, using a NANOSENSORS™ AdvancedTEC™ ATEC-NC tip-view AFM probe.* https://www.nanosensors.com/advanced-tip-at-the-end-of-the-… Please have a look at the NANOSENSORS blog for the full citation and a direct link to the full article. #AFMプローブ #AtomicForceMicroscopy #走査型走査型プローブ顕微鏡

Happy and Prosperous 2020 from the nanotools Team!Wed Jan 08 2020

Happy and Prosperous 2020 from the nanotools Team!

Have fun! All the best to you in 2020!Mon Dec 30 2019

Merry Christmas and happy 2020 from BudgetSensors®Mon Dec 23 2019

Season's Greetings from the whole NanoWorld team!Mon Dec 23 2019

New post - Season’s Greetings from the whole NanoWorld® team! (https://www.nanoworld.com/…/seasons-greetings-from-the-who…/) has been published on NanoWorld® Blog. The whole team at NanoWorld AFM probes wishes you Merry Christmas and a Happy New Year! Merry Christmas and a Happy New Year! #HappyHolidays #AFMprobes #SeasonsGreetings2019 #nanoscience #AFMプローブ