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Dielectric Properties of Polymer Nanocomposite Interphases Using Electrostatic Force Microscopy and Machine LearningMon Jul 22 2024

Metrology for measuring the interfacial dielectric permittivity in dielectrics with Electrostatic Force Microscopy (EFM) measurements and machine learning. AFM measurements performed using our platinum coated ElectriMulti75-G AFM probes.

NanoWorld Interfacial water on collagen nanoribbons by 3D AFM - news

Interfacial water on collagen nanoribbons by 3D AFMMon Jul 22 2024

Interfacial water on collagen nanoribbons by 3D AFM
Collagen is the most abundant structural protein in mammals. * Type I collagen in its fibril form has a characteristic pattern structure that alternates two regions called gap and overlap. The structure and properties of collagens are highly dependent on the water and mineral content of the environment. * In the article “Interfacial water on collagen nanoribbons by 3D AFM” Diana M. Arvelo, Clara Garcia-Sacristan, Enrique Chacón, Pedro Tarazona and Ricardo Garcia describe how they apply three dimensional atomic force microscopy (3D AFM) to characterize at angstrom-scale resolution the interfacial water structure of collagen nanoribbons.* Three-dimensional AFM (3D AFM) is […]

NANOSENSORS Magnetic control of self-assembly and disassembly in organic materials - news

Magnetic control of self-assembly and disassembly in organic materialsWed Jul 10 2024

#Magneticallyresponsivematerials are highly valuable for biological and medical uses because the magnetic field is non-invasive, radiation-free, and safe for humans.*
DNA and some proteins have also been shown to be magnetically responsive; however, with very few exceptions, an extremely strong magnetic field is typically required to influence the behaviours of such #biomolecules.*
As organic molecules and materials are generally insensitive or weakly sensitive to magnetic fields, it is necessary to find ways and means to increase their #magneticresponsiveness. *
Because #magneticsusceptibility is additive, one possible way of increasing magnetic responsiveness is to place multiple magnetically responsive moieties within a single molecule in a favourable orientation.
The hashtag#αhelix, one of the most common secondary structures of #proteins, provides a nice example of constructive addition in diamagnetism.
In the article “Magnetic control of self-assembly and disassembly in organic materials” You-jin Jung, Hyoseok Kim, Hae-Kap Cheong and Yong-beom Lim show that the magnetic control of assembly and disassembly under the ordinary magnetic field strength is possible by synergistically combining the concepts of the perfect α-helix and the #rodcoil #supramolecularbuildingblocks.*
You-jin Jung et al. experimentally verify the magnetic responsiveness both in solution state and in thin films containing equilibrium morphologies.*
To make this possible, the key prerequisite was the development of the α-helical peptide, which has multiple characteristics simultaneously.*
The authors show that the #selfassembly processes of the designed rod-coils and #disassembly of rod-coil/DNA complexes can be controlled in a magnetically responsive manner using the relatively weak magnetic field provided by the ordinary neodymium magnet [0.07 ~ 0.25 Tesla (T)].*
These results demonstrate that magnetically responsive #organicassemblies usable under practical conditions can be realized by using rod-coil supramolecular building blocks containing constructively organized diamagnetic moieties.*
This study lays foundation to magnetically interface organic materials with magnetic devices and instruments, with application potentials in magnetically responsive #bionanomaterials, molecular magnetic devices, and smart peptide/nucleic acid complexes highly responsive to magnetic field.*
The morphological states were characterized by #atomicforcemicroscopy (AFM) and transmission electron microscopy (TEM).
#AFM was performed with a commercially available #atomicforcemicroscope in non-contact mode with NANOSENSORS™ PointProbe® Plus PPP-NCHR #AFMprobes.
The full citation and a direct link to the full article can be found in the NANOSENSORS™ blog:…/magnetic-control-of-self…/

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MikroMasch® HQ:NSC35/tipless/No Al AFM cantileversFri Jul 05 2024

Our customers have glued a number of different objects on the MikroMasch HQ:NSC35/tipless/No Al AFM cantilevers, including microspheres and custom AFM tips, but this is the first time we know of when our cantilevers are used to hold the AFM sample. Check out this work on reverse tip sample Atomic Force Microscopy enabled by AFM probe chip nanofabrication!

NanoWorld Stiffness Mediated-Mechanosensations of Airway Smooth Muscle Cells on Linear Stiffness Gradient Hydrogels - news

Stiffness Mediated-Mechanosensations of Airway Smooth Muscle Cells on Linear Stiffness Gradient HydrogelsThu Jul 04 2024

Stiffness Mediated-Mechanosensations of Airway Smooth Muscle Cells on Linear Stiffness Gradient Hydrogels
Airflow limitation in obstructive airway disease is characterized by narrowing of the airway lumen from excessive contraction of airway smooth muscle (ASM) and remodeling of the airway wall which includes changes in the extracellular matrix (ECM) of the ASM layer.* Previous studies on human airway smooth muscle cells ( hASMC ) have independently assessed the influence of extracellular matrix (ECM) proteins on substrates of supra-physiological stiffnesses, such as tissue culture plastic or glass.* While the influence of discrete substrate stiffness on hASMC behavior has been examined, manipulation of both substrate stiffness and ECM proteins simultaneously (as expected in disease) has […]

NanoWorld Meet NanoWorld® at NanoKorea 2024 - news

Meet NanoWorld® at NanoKorea 2024Wed Jul 03 2024

NanoWorld AG CEO Manfred Detterbeck is attending NANOKOREA 2024, the 22nd International #Nanotech Symposium & Exhibition on nanoscale science and technology , which will be held from July 3-5, 2024 at KINTEX (Korea International Exhibition Center), Goyang-si, Gyeonggi-do, South Korea.

This year’s main theme is: Invisible Nano Realizes the Future

Will we meet you there too?

NANOSENSORS Teaching an old dog new tricks: Ti-doped ZnFe2O4 as active material in zinc ion batteries – a proof of concept - news

Teaching an old dog new tricks: Ti-doped ZnFe2O4 as active material in zinc ion batteries – a proof of conceptMon Jul 01 2024

#ZnFe2O4 is a well-investigated, versatile material with a spinel structure, which reportedly shows electrical conductivity in the range of 5 to 10 mS cm−1, a relatively small electronic bandgap of about 1.9 eV and values of 2.02 eV and 2.33 eV for the indirect and direct optical band gap. *
Apart from the low production price, the high and globally uniform availability of the constituents, high chemical stability against air or moisture and the harmlessness with regard to health and the environment, these characteristics make it an ideal candidate for photocatalytic or energy harvesting applications. *
In addition, #nanostructured ZnFe2O4 has gained interest because depending on the size of the #nanostructure and the synthesis method, Zn and Fe can partially exchange sites in the crystal structure. The degree of cation exchange allows tuning of the electronic and spin structure of the material making it interesting for various uses ranging from spintronic and microwave applications to sensor materials. *
In the field of #energystorage , ZnFe2O4 has previously been discussed as possible material for the negative electrode in lithium-ion batteries (LIB). Even though its high theoretical capacity of 1072 A h kg−1 as well as its low toxicity compared to conventional Co- and Ni-containing compounds and the low production costs make it highly attractive, further investigations showed that the cycle stability is less than ideal, because the ZnFe2O4 breaks down to ZnO and Fe2O3 during repeated charging and discharging in a cell and metal ion dissolution takes place. *
In addition, the energy efficiency is very low. Still, various studies have been conducted that either condone decomposition since even the decay products still work well as negative electrode or do not charge and discharge over the full voltage window (shallow cycling) to prevent decomposition. *
In the article “Teaching an old dog new tricks: Ti-doped ZnFe2O4 as active material in zinc ion batteries – a proof of concept” Susanna Krämer, Julia Hopster, Anna Windmüller, R.-A. Eichel, M. Grünebaum, T. Jüstel, M. Winter and Kerstin Neuhaus investigate the suitability of the spinel material ZnFe2O4 for use as active material for the cathode side in #zincionbatteries. *
In addition to pure ZnFe2O4, part of the Fe3+ was doped with Ti4+ to achieve stabilization of Zn vacancies in the material and increase ionic conductivity as indicated by previous modelling results. *
For their study Susanna Krämer et al. produced samples with the compositions ZnFe2−xTixO4 (with x = 0 to 0.25) by a Pechini synthesis route. The crystal structure, microstructure, optical and electrochemical characteristics of the material were analyzed and compared to literature results. *
It has been successfully demonstrated that both pure and Ti doped ZnFe2O4 can be used as active material in the positive electrodes of zinc metal batteries or in an ‘‘anode-free’’ setup with Sn metal. *
Cells with calcined ZnFe2xTixO4 (x = 0.09)|0.5 M zinc triflate in acetonitrile|Zn showed a stable cycling behavior over 1000 cycles and an average initial specific capacity of 55 mA h g1.*
In their study the authors found pure #zincferrite and material with low Ti concentrations (specifically x = 0.09) to work as active material in ZIBs. Samples above the solubility limit of Ti (in our case x = 0.13) did not show a stable cycling behavior. Addition of higher amounts of Ti was hence not deemed favorable to improve application of ZnFe2O4 as active material in zinc batteries. *
From studies on the use of ZnFe2O4 and ZnMn2O4 in various electrochemical applications, a variety of synthesis methods are already known for micro-structuring the material in order to increase the surface area and enable more effective use, ideally in combination with an electron conductive coating. This is a future target to considerably improve performance, especially the capacity, of ZnFe2O4-based materials for use in zinc metal and zinc ion batteries. *
#KelvinProbeForceMicroscopy (#KPFM) measurements of ZFO, ZFTO7 and ZFTO13 showed a slight decrease in work function with increasing Ti content (Fig. 5a cited below). In addition, it was observed that for all measured samples, the grain boundaries showed a lower surface potential compared to the grain interiors (Fig. 5b). The difference was evaluated for 25 different grain boundary position in the three samples, but the potential difference for the samples did not depend on the Ti concentration and was roughly in the range of 25 mV, but with a large variation between individual grain boundaries (cf. Fig. S2, ESI†). It can therefore be assumed that this difference is merely due to band bending, which can typically be found at grain boundaries due to the different crystallographic orientation of adjacent grains. *
Kelvin Probe Force Microscopy (KPFM) measurements were performed in an Ar atmosphere using a commercially available atomic force microscopy with conductive NANOSENSORS PointProbe® Plus PPP-NCSTPt #AFMtips. *…
The samples were used as received without further surface modification and were dried in an Ar stream in the instrument before starting the measurements. KPFM measurements yield data about the local surface potential of a sample, which under ideal conditions is directly related to the Volta potential. If Ti4+ works as an n-type dopant in ZnFe2O414 an increasing work function (which means a decreasing surface potential) with increasing Ti4+ content can be expected.
Before and after the measurements, the AFM tip was calibrated on a freshly cleaved highly ordered pyrolytic graphite reference surface to minimize influence of AFM tip wear on the results. *
Please have a look at the NANOSENSORS blog for the full citation and a direct link to the full article:

NanoWorld Highly efficient carbon-dot-based photoinitiating systems for 3D-VAT printing - news

Highly efficient carbon-dot-based photoinitiating systems for 3D-VAT printingThu Jun 27 2024

Highly efficient carbon-dot-based photoinitiating systems for 3D-VAT printing
Known as a rising star among carbon nanomaterials, carbon dots (CDs) have attracted considerable interest in various fields in recent years.* In the article “Highly efficient carbon dot-based photoinitiating systems for 3D-VAT printing” Dominika Krok, Wiktoria Tomal, Alexander J. Knight, Alexander I. Tartakovskii, Nicholas T. H. Farr, Wiktor Kasprzyk and Joanna Ortyl describe how they synthesized different types of carbon dots (CDs) based on citric acid as a precursor using an efficient procedure to purify these materials from low molecular by-products and fluorophores.* They introduce three types of CDs: citric acid-based, as well as ammonia- and ethylenediamine-doped CDs, and compare […]

nanotools Studying the Interaction of Engineered Liposomes with Living Cells - news

Studying the Interaction of Engineered Liposomes with Living CellsTue Jun 25 2024

Discover how nanotools biosphere™ with 2µm radius are applied to analyze adhesion forces and Young’s modulus in HeLa cells.
  • Title: On the uptake of cationic liposomes by cells: From changes in elasticity to internalization
  • DOI: 10.1016/j.colsurfb.2022.112968
  • Authors: Adrià Botet-Carreras, Manel Bosch Marimon, Ruben Millan-Solsona, Eva Aubets, Carlos J. Ciudad, Véronique Noé, M. Teresa Montero, Òscar Domènech, Jordi H. Borrell
  • Publication: Colloids and Surfaces B: Biointerfaces
  • Publisher: Elsevier
  • Date: January 2023

NanoAndMore NanoAndMore  Japan will be exhibiting at the 21st International Congress of Biophysics

NanoAndMore Japanは、第21回国際生物物理学会議にブース番号7にて出展いたしますMon Jun 24 2024

NanoAndMore Japanは、第21回国際生物物理学会議にブース番号7にて出展いたします。@IUPAB2
2024年6月24日~28日 国立京都国際会館


NanoAndMore Japan will be exhibiting at the 21st International Congress of BiophysicsMon Jun 24 2024

NanoAndMore Japan will be exhibiting at the 21st International Congress of Biophysics at booth number 7. International Union of Pure and Applied Biophysics
June 24 - 28, 2024, Kyoto International Conference Center
Please feel free to stop by.
The huge AFM cantilever is a landmark!
Find out more about the about the big and the small #AFMprobes that we offer.

NanoWorld Join us at the Panel Discussion on SPM Cantilevers at SPM Connect today - news

Join us at the Panel Discussion on SPM Cantilevers at SPM Connect todayTue Jun 18 2024

Join us at the Panel Discussion on SPM Cantilevers at SPM Connect today
It’s the second day at #SPMConnect in Washington DC. There is a Panel Discussion on #SPMCantilevers today at 3:30PM – National Harbor 5. Our colleagues Manfred Detterbeck (NanoWorld CEO) and Dr. Oliver Krause (NanoWorld R&D team) will be there. Come and join us! We are looking forward to your questions and an interesting discussion.

NANOSENSORS Distinct Contact Guidance Mechanisms in Single Endothelial Cells and in Monolayers - news

Distinct Contact Guidance Mechanisms in Single Endothelial Cells and in MonolayersThu Jun 13 2024

In many #tissues, #cellshape and orientation are controlled by a combination of internal and external #biophysicalcues. *
Anisotropic #substratetopography is an ubiquitous cue that leads to cellular elongation and alignment, a process termed #contactguidance whose underlying mechanisms remain incompletely understood. *
In medium and large #arteries, atherosclerotic lesions initiate preferentially near branches and bifurcations where the #endothelialcells (ECs) lining the blood vessels are cuboidal and randomly oriented. *
In contrast, zones where endothelial cells ECs are elongated and aligned in the direction of blood flow tend to be protected from the disease. *
These observations suggest a prominent shape-function relationship and motivate interest in elucidating the mechanisms governing the regulation of EC morphology and alignment. *
In their article “Distinct Contact Guidance Mechanisms in Single Endothelial Cells and in Monolayers” Claire Leclech, Apoorvaa Krishnamurthy, Laurent Muller and Abdul I. Barakat seek to fill some of the remaining gaps in the understanding of endothelial cells shape regulation by microgrooves.
The authors try to find out, whether contact guidance responses are similar in single cells and in cellular monolayers.
To this end Leclech et al. used microgrooved substrates to investigate the contact guidance response of #vascularendothelialcells (ECs) at densities ranging from single cells at an early stage of culture as well at a later stage when the cells attain confluence, form monolayers, and exhibit collective behavior. *
The fact that the authors follow the contact guidance response of ECs in time, from #singlecells to the formation and maturation of #monolayers is the main novelty of their article. *
By tracking EC shape on #microgrooves in time and with increasing cell density, Claire Leclech et al. found that #cellalignment and elongation progressively decrease, which they interpret as a gradual loss of response to substrate topography.*
The authors could show that contrary to common belief, focal adhesion (FAs) are not the principal elements involved in the prominent groove depth-dependent contact guidance in single ECs.*
Interestingly, the contact guidance response is greatly attenuated in confluent monolayers, and cell shape and alignment in that case are driven by the organization of the #basementmembrane (BM) secreted by the cells, which leads to a loss of cellular interaction with the microgrooves.
For the characterization of the secreted basment membrane the #surfacetopography and #stiffness of the different samples were determined by #Atomicforcemicroscopy ( #AFM) with an AFM mounted on a fluorescent microscope. *
NANOSENSORS uniqprobe qp-BioAC-CI #AFMprobes with circular symmetric rounded #AFMtips with a typical tip radius of 30 nm were used.
The full citation and a direct link to the full article can be found in the NANOSENSORS blog:

NanoWorld A beginner’s guide to the Characterization of Hydrogel Microarchitecture for Cellular Applications - news

A beginner’s guide to the Characterization of Hydrogel Microarchitecture for Cellular ApplicationsThu Jun 06 2024

A beginner’s guide to the #Characterization of #Hydrogel #Microarchitecture for Cellular Applications
Hydrogel materials show a number of properties which makes them interesting candidates to be utilized to mimic the #extracellularmatrix (ECM). Therefore, these materials are attractive for use in biological applications such as tissue engineering, cell culture 3D bioprinting and more.
Are you planning to use #hydrogels for the first time in your research?
Then have a look at the insightful article “A beginner’s guide to the Characterization of Hydrogel Microarchitecture for Cellular Applications” by Francisco Drusso Martinez-Garcia, Tony Fischer, Alexander Hayn, Claudia Tanja Mierke, Janette Kay Burgess and Martin Conrad Harmsen.
In their article the authors describe and evaluate the different technologies that are most commonly used to assess hydrogel microarchitecture.
Francisco Drusso Martinez-Garcia et al. explain the working principle of the various methods and also discuss the merits and limitations of each of them in view of their usefulness for the characterization of hydrogels.
Atomic force microscopy ( #AFM) can be used to investigate the hydrogel #surfacetopology as well as a hydrogel’s #mechanicalproperties. The latter can be achieved through mathematical modelling of #forcedistancecurves.
When using the AFM to characterize the elasticity of a hydrogel sample it is essential to take the stiffness of the investigated material into account when choosing what kind of #AFMprobe to use for these experiments.
If an #AFMcantilever used for probing a soft sample is too stiff (if the force constant/spring constant is too high) this might result in a poor signal-to-noise ratio.
If a soft AFM probe (an AFM probe with an AFM cantilever with a low force constant) is chosen to investigate a soft material this should lead to a better signal-to-noise ratio. On the other hand, if an AFM cantilever is too soft (if the force constant is too low) then it might not be stiff enough to indent the investigated material.
Another critical factor is the shape and the size of the AFM tip.
Spheroidal AFM probes might stick to the material, resulting in artefacts, disrupted force–distance curves, or even damaged AFM cantilevers. If the #AFMtip is much smaller than the pore size of the hydrogel, it might get stuck in the fibrous network microarchitecture.
On the other hand, if the spherical AFM tip, e.g. as in #colloidalAFMprobes (a sphere glued to end of a tipless AFM cantilever), is too large, the weight of the sphere can have a negative influence on the spring characteristics of the AFM cantilever.
All these factors and more as described in the cited article have to be carefully weighed before deciding on the settings of the atomic force microscope and choosing an AFM probe for the investigation of a specific hydrogel.
@NanoWorld tipless ArrowTL2 cantilever arrays with polystyrene beads glued to them were used by the authors of this beginner’s guide to achieve the AFM data presented in the article.*
Please have a look at the NanoWorld blog for the full citation and a direct link to the full article.

NANOSENSORS Room-temperature sub-100 nm Néel-type skyrmions in non-stoichiometric van der Waals ferromagnet Fe3-xGaTe2 with ultrafast laser writability - news

Room-temperature sub-100 nm Néel-type skyrmions in non-stoichiometric van der Waals ferromagnet Fe3-xGaTe2 with ultrafast laser writabilityWed May 29 2024

The small size and magnetoelectronic properties of #magneticskyrmions make them promising candidates as data carriers for future high-density and fast-speed data storage, quantum and neuromorphic computation. *
Because of their unique atomic layered structure, two-dimensional (2D) van der Waals (vdW) #ferromagnets have some advantages when using them for practical applications in spintronic devices compared to 3D bulk ferromagnets. *
Therefore, the study of magnetic #skyrmions with small sizes, especially at room temperature (RT), in 2D vdW #ferromagneticmaterials with easy tunability has become a focal point of magnetic and topological order of matters, as well as spintronic applications. *
In the article “Room-temperature sub-100 nm Néel-type skyrmions in non-stoichiometric van der Waals ferromagnet Fe3-xGaTe2 with ultrafast laser writability” Zefang Li, Huai Zhang, Guanqi Li, Jiangteng Guo, Qingping Wang, Ying Deng, Yue Hu, Xuange Hu, Can Liu, Minghui Qin, Xi Shen, Richeng Yu, Xingsen Gao, Zhimin Liao, Junming Liu, Zhipeng Hou, Yimei Zhu and Xuewen describe how they discovered that the iron deficiency in Fe3GaTe2 can lead to a pronounced displacement of the Fe atoms within the crystal structure.*
Based on systematic structural analysis and first-principles calculations, Zefang Li et al. find that this #atomicdisplacement causes a transformation from the original centrosymmetric crystal structure to a non-centrosymmetric structure, resulting in significant Dzyaloshinskii–Moriya interactions (DMI). *
They report the discovery of a field-free sub-100 nm #Néeltypeskyrmion state in non-stoichiometric Fe2.84±0.05GaTe2 over a broad temperature range from 330 K to 100 K. *
Lorentz transmission electron microscopy (LTEM) (along with along with #magneticforcemicroscopy ( #MFM ) shows the size of the skyrmions decreases with sample thickness, and a field-free sub-100 nm skyrmion state was achieved at RT within a specific sample thickness range of 40 nm to 60 nm. *
Magnetic force microscopy (MFM), which is sensitive to the out-of-plane magnetic field in the sample, was used by Zefang Li et al. to study the effect of #nanoflake thickness on the stabilization of the #skyrmionphase at room temperature after field-cooling manipulation. *
All the nanoflakes used for the magnetic force microscopy (MFM) measurements were freshly exfoliated from the same batch to ensure consistency. *
In order to minimize the influence of the #AFMtip’s magnetic field on the #domainstructures during scanning the authors used NANOSENSORS PPP-LM-MFMR low-moment magnetic #AFMprobes.
These #MFMprobes are designed for magnetic force microscopy with reduced disturbance of the magnetic sample by the AFM tip and enhanced lateral resolution – compared to the standard MFM probes .*
The MFM measurements were carried out in an environment continuously flushed with argon gas to ensure effective protection.*
To further enhance the practical applications of their findings, Zefang Li et al. employed a homemade in-situ optical Lorentz transmission electron microscope to demonstrate ultrafast writing of skyrmions in Fe3-xGaTe2 using a single femtosecond laser pulse.*
The article by Zefang Li et al. demonstrates not only the non-stoichiometric Fe2.84±0.05GaTe2 to be a promising material platform for exploring magnetic skyrmions, but also that fs-laser can be a powerful tool to manipulate and control topological chiral spin textures to realize skyrmion-based high-speed logic and memory applications.*
Please have a look at the NANOSENSORS blog for the full citation and a direct link to the full article.

NanoWorld Flexible Polyurethane Foams Modified with Novel Coconut Monoglycerides-Based Polyester Polyols - news

Flexible Polyurethane Foams Modified with Novel Coconut Monoglycerides-Based Polyester PolyolsFri May 24 2024

Flexible Polyurethane Foams Modified with Novel Coconut Monoglycerides-Based Polyester Polyols
The products of the polyurethane (PU) industry such as foams, coatings and adhesives are numerous and can be found in many areas of everyday life. * Polyols are an essential component in the production of polyurethane. Nowadays they mostly come from petroleum products. * In view of potential risk factors such as the running out of fossil fuels, supply chain issues, environmental concerns and economic risks it is important to develop alternatives as substitutes and supplements to the existing petroleum derived polyols. * Vegetable oils can be used to manufacture biobased polyols and various oils such as linseed oil, rapeseed […]

nanotools Understanding Cardiac Dysfunction in Arrhythmogenic Cardiomyopathy - news

Understanding Cardiac Dysfunction in Arrhythmogenic CardiomyopathyThu May 23 2024

Publication update:
nanotools biotool cell XXL featured in research article

Discover how nanotools 15 µm long, conical biotool cell XXL with 25 nm radius was applied for nanomechanical studies of live human cardiac cells.

  • Title: SARS-CoV-2 protein Nsp1 alters actomyosin cytoskeleton and phenocopies arrhythmogenic cardiomyopathy-related PKP2 mutant
    DOI: 10.1101/2020.09.14.296178
  • Authors: Cristina Márquez-López, Marta Roche-Molina, Nieves García-Quintáns, Silvia Sacristán, David Siniscalco, Andrés González-Guerra, Emilio Camafeita, Mariya Lytvyn, María I. Guillen, David Sanz-Rosa, Daniel Martín-Pérez, Cristina Sánchez-Ramos, Ricardo García, Juan A. Bernal
  • Publication: bioRxiv
  • Publisher: Cold Spring Harbor Laboratory
  • Date: September 16, 2020

NANOSENSORS Lipoplex-Functionalized Thin-Film Surface Coating Based on Extracellular Matrix Components as Local Gene Delivery System to Control Osteogenic Stem Cell Differentiation - news

Lipoplex-Functionalized Thin-Film Surface Coating Based on Extracellular Matrix Components as Local Gene Delivery System to Control Osteogenic Stem Cell DifferentiationThu May 16 2024

The development of smart multifunctional #biomaterials with the ability to control the behavior of #stemcells on demand has become a powerful strategy in regenerative medicine and cell therapies.*
Stem cell-based therapies for example can offer possibilities to regenerate critical size bone defects from severe fractures or bone tissue loss after surgery. *
Various studies have determined characteristics and modifications of biomaterials that enable initiation of stem cell osteogenesis and represent promising approaches for clinical use. These approaches include materials that can mimic the bone microenvironment, materials with specific mechanical properties which stimulate bone tissue formation, and materials which can release or control the activity of osteoinductive growth factors. *
A straightforward strategy for the #functionalization of biomaterials involves emulating the properties of the #extracellularmatrix (ECM) for the formation of an artificial microenvironment that enables a precise control of cell behavior and function. *
Due to interaction of ECM components with cell surface receptors such as integrins, ECM regulates cell proliferation, migration, and differentiation.*
Therefore, the development of #surfacecoatings mimicking the native ECM structure and function is of considerable interest to functionalize implant materials. *
The #layerbylayer (LbL) technology, well-known from the pioneering work by Decher et al. on the development of #polyelectrolytemultilayers (PEMs) on solid surfaces by alternating deposition of oppositely charged polyelectrolytes, has evolved into a very simple and cost effective yet highly versatile and efficient surface modification and functionalization technology.*
The LbL technique allows the production of multifunctional thin film coatings with precise control of the film composition, structure, properties, and functions at the nanoscale. *
A further advantage of LbL is that it can be performed by different methods such as dip-coating, spray coating, and spin-coating protocols allowing the coating of different materials and designs also in a time-saving manner.*
Indeed, polyelectrolyte multilayers (PEMs) have been broadly used as reservoir for either the surface immobilization or encapsulation of bioactive molecules, more precisely drugs and proteins, to engineer bio-functional materials by choice of polyelectrolytes and complexation conditions for regenerative medicine strategies.*
Previous studies have consistently demonstrated that electrostatic-driven LbL assembly is a powerful and simple technique to functionalize biomaterials with nucleic acids aiming for non-viral gene delivery. *
In the article “Lipoplex-Functionalized Thin-Film Surface Coating Based on Extracellular Matrix Components as Local Gene Delivery System to Control Osteogenic Stem Cell Differentiation” Catharina Husteden, Yazmin A. Brito Barrera, Sophia Tegtmeyer, João Borges, Julia Giselbrecht, Matthias Menzel, Andreas Langner, João F. Mano, Christian E. H. Schmelzer, Christian Wölk and Thomas Groth describe how they combined both approaches, such as the ECM-mimicking character of PEM and their ability to be used as carrier for in situ transfection, to develop a gene-activated ECM-mimicking surface coating to direct stem cells’ fate.*
They focused on a bone ECM-mimicking PEMs consisting of Col and Cs loaded with LPX composed of OO4/DOPE lipid composite. *
Furthermore, they studied the ability of the system to induce osteogenic stem cell differentiation by gene expression analysis and mineralization assays. *
The authors present a new approach to engineer a bone-ECM inspired gene-activated surface coating which allows controlling stem cells function, and consequently, represents a promising tool to develop multifunctional surface coatings for regenerative medicine strategies. *
#AtomicForceMicroscopy ( #AFM ) was used to study the #topography and the #mechanicalproperties of the different polyelectrolyte multilayers (PEMs). *
In order to investigate the #elasticity and the #forcecurve, the samples were compressed by the #AFMtip and the #forcemapping mode was applied while the AFM tip scanned a specific area of the sample. *
The #forcemappingmode measured the #interactionforces such as adhesion or electrostatics and gives an idea regarding the stiffness and topography. The interest in testing mechanical properties and topography of surface coatings is related to their effect on #cellbehavior, such as spreading, proliferation, and differentiation. *
Atomic force microscopy with a commercially available AFM instrument in combination with an inverted fluorescence microscope was performed in #quantitativeimaging mode (QITM1) to investigate the #surfaceroughness and topography as well as record corresponding fluorescence images. *
The topographical images were recorded using @NANOSENSORS uniqprobe qp-BioT #AFMcantilevers in a standard liquid cell containing 0.15 m NaCl solution. The uniqprobe qp-BioT AFM probe types with their two different triangular AFM cantilevers (Cantilever Beam 1 (CB1) typical resonant frequency 50 kHz, typical force constant 0.3 N/m, Cantilever Beam 2 (CB2): typical resonant frequency 20 kHz, typical force constant 0.08 N/m ) offer an alternative to silicon nitride #AFMprobes, with the advantage of reduced thermal drift and taller AFM tips with smaller opening angles. *
A force map area of 5 × 5 µm2 was recorded with a resolution of 512 × 512 pixel2.
Please have a look at the NANOSENSORS blog for the full citation and a direct link to the full article
¹QITM mode is a trademark of Bruker Nano GmbH